Differential allelic representation (DAR) identifies candidate eQTLs and improves transcriptome analysis

In comparisons between mutant and wild-type genotypes, transcriptome analysis can reveal the direct impacts of a mutation, together with the homeostatic responses of the biological system. Recent studies have highlighted that, when the effects of homozygosity for recessive mutations are studied in non-isogenic backgrounds, genes located proximal to the mutation on the same chromosome often appear over-represented among those genes identified as differentially expressed.

Citation:
Baer L, Barthelson K, Postlethwait JH, Adelson DL, Pederson SM, Lardelli M. Differential allelic representation (DAR) identifies candidate eQTLs and improves transcriptome analysis. PLoS Comput Biol. 2024;20

Keywords:
RNA; Co-located; Differentially expressed gene; Direct impact;
Isogenic; Quantitative trait locus; Transcriptome analysis; Wild types; Genes

Abstract:
In comparisons between mutant and wild-type genotypes, transcriptome analysis can reveal the direct impacts of a mutation, together with the homeostatic responses of the biological system. Recent studies have highlighted that, when the effects of homozygosity for recessive mutations are studied in non-isogenic backgrounds, genes located proximal to the mutation on the same chromosome often appear over-represented among those genes identified as differentially expressed.