Patient-Derived Orthotopic Xenograft Models for High-Grade Pediatric Brain Cancers

Patient-derived orthotopic xenograft (PDOX) mouse models are considered the gold standard for evidence-based preclinical research in pediatric neuro-oncology. This protocol describes the generation of PDOX models by intracranial implantation of human pediatric brain cancer cells into immune-deficient mice, and their continued propagation to establish cohorts of animals for preclinical research.

Citation:
Buck J, Hii H, Endersby R. Patient-Derived Orthotopic Xenograft Models for High-Grade Pediatric Brain Cancers. Methods Mol Biol. 2024;2806:41-53.

Keywords:
Mouse models of cancer; Patient-derived xenograft; Pediatric brain cancer; Preclinical models

Abstract:
Patient-derived orthotopic xenograft (PDOX) mouse models are considered the gold standard for evidence-based preclinical research in pediatric neuro-oncology. This protocol describes the generation of PDOX models by intracranial implantation of human pediatric brain cancer cells into immune-deficient mice, and their continued propagation to establish cohorts of animals for preclinical research.