Single-cell transcriptomic and spatial landscapes of the developing human pancreas

Current differentiation protocols have not been successful in reproducibly generating fully functional human beta cells in vitro, partly due to incomplete understanding of human pancreas development. Here, we present detailed transcriptomic analysis of the various cell types of the developing human pancreas, including their spatial gene patterns. We integrated single-cell RNA sequencing with spatial transcriptomics at multiple developmental time points and revealed distinct temporal-spatial gene cascades.

Citation:
Olaniru OE, Kadolsky U, Kannambath S, Vaikkinen H, et al. Single-cell transcriptomic and spatial landscapes of the developing human pancreas. Cell Metab. 2023;35(1):184-99.e5

Keywords:
Schwann cells; Visium; beta cell development; endocrine progenitors; human fetal pancreas; scRNA-seq; spatial transcriptomics; trajectory inference

Abstract:
Current differentiation protocols have not been successful in reproducibly generating fully functional human beta cells in vitro, partly due to incomplete understanding of human pancreas development. Here, we present detailed transcriptomic analysis of the various cell types of the developing human pancreas, including their spatial gene patterns. We integrated single-cell RNA sequencing with spatial transcriptomics at multiple developmental time points and revealed distinct temporal-spatial gene cascades.