Copy number variation in tRNA isodecoder genes impairs mammalian development and balanced translation

The number of tRNA isodecoders has increased dramatically in mammals, but the specific molecular and physiological reasons for this expansion remain elusive. To address this fundamental question we used CRISPR editing to knockout the seven-membered phenylalanine tRNA gene family in mice, both individually and combinatorially.

Citation:
Hughes LA, Rudler DL, Siira SJ, ……. Rackham O, Filipovska A. Copy number variation in tRNA isodecoder genes impairs mammalian development and balanced translation. Nat Commun. 2023;14(1)

Keywords:
Transfer RNA; copy number variation; genetics; mammal; metabolism; DNA Copy Number Variations

Abstract:
The number of tRNA isodecoders has increased dramatically in mammals, but the specific molecular and physiological reasons for this expansion remain elusive. To address this fundamental question we used CRISPR editing to knockout the seven-membered phenylalanine tRNA gene family in mice, both individually and combinatorially.