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ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing

Canonical RNA processing in mammalian mitochondria is defined by tRNAs acting as recognition sites for nucleases to release flanking transcripts. The relevant factors, their structures, and mechanism are well described, but not all mitochondrial transcripts are punctuated by tRNAs, and their mode of processing has remained unsolved.

Citation:
Clemente P, Calvo-Garrido J, Pearce SF, ……….. Rackham O, Filipovska A, Rorbach J, Freyer C, Wredenberg A. ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing. Nature Communications. 2022;13(1)

Keywords:
Mitochondrial protein; mitochondrial RNA; phosphatase; carbon; phosphate; confocal microscopy; Drosophila; enzyme activity; hydrolysis; immunohistochemistry; immunoprecipitation; in vitro study; in vivo study; liquid chromatography-mass spectrometry; mitochondrial gene; mitochondrion; proteomics; everse transcription polymerase chain reaction

Abstract:
Canonical RNA processing in mammalian mitochondria is defined by tRNAs acting as recognition sites for nucleases to release flanking transcripts. The relevant factors, their structures, and mechanism are well described, but not all mitochondrial transcripts are punctuated by tRNAs, and their mode of processing has remained unsolved.