Optimization of blood handling and peripheral blood mononuclear cell cryopreservation of low cell number samples

Rural/remote blood collection can cause delays in processing, reducing PBMC number, viability, cell composition and function. To mitigate these impacts, blood was stored at 4◦ C prior to processing. Viable cell number, viability, immune phenotype, and Interferon-γ (IFN-γ) release were measured.

Citation:
Hope CM, Huynh D, Wong YY, Oakey H, Perkins GB, …… Binkowski S, Bui M, Choo AYL, et al. Optimization of blood handling and peripheral blood mononuclear cell cryopreservation of low cell number samples. Int J Mol Sci. 2021;22(17).

Keywords:
Blood handling; Cell concentration; Cryopreservation; Delay in processing; PBMC

Abstract:
Rural/remote blood collection can cause delays in processing, reducing PBMC number, viability, cell composition and function. To mitigate these impacts, blood was stored at 4◦ C prior to processing. Viable cell number, viability, immune phenotype, and Interferon-γ (IFN-γ) release were measured.