Detection of occult Scedosporium species in respiratory tract specimens in cystic fibrosis (CF) by use of selective media

Authors:
Blyth CC, Harun A, Middleton PG, Sleiman S, Lee O, Sorrell TC, Meyer W, Chen SCA

Authors notes:
Journal of Clinical Microbiology 2010; 48(1): 314-316. (IF = 4.162; CI = 5)

Keywords:
detection, occult Scedosporium species, respiratory tract specimens, cystic fibrosis, CF, selective media, respiratory samples, systic fibrosis outpatients, Sabouraud's dextrose agar, SABD, antibiotics, Mycosel, Scedosporium-selective medium, SceSel+

Abstract:
Respiratory samples from cystic fibrosis outpatients were cultured on Sabouraud's dextrose agar (SABD) containing antibiotics, Mycosel, and Scedosporium-selective medium (SceSel+). Thirty-two (14.7%) of 218 specimens from 11/69 (15.9%) patients yielded a Scedosporium sp., most frequently Scedosporium aurantiacum (17/218). Scedosporium was recovered on SceSel+, Mycosel, and SABD from 90.6%, 50.0%, and 46.9% of the specimens tested, respectively.