Authors:
Beesley AH, Weller RE, Senanayake S, Welch M, Kees UR
Authors notes:
Leuk Res. 2009 Feb;33(2):321-5. Epub 2008 Sep 11.
Keywords:
Receptor mutation, not a common mechanism, naturally occurring glucocor ticoid resistance, leukaemia cell lines
Abstract:
Glucocorticoids (GCs) are among the most important drugs for the treatment of acute lymphoblastic leukaemia (ALL). Cell lines cultured in high GC concentrations typically contain mutated glucocorticoid receptor (GR), something that is rarely found in primary ALL specimens. We studied naturally occurring mechanisms of GC resistance and examined sensitivity to GC in 15 T-ALL cell lines grown without prior exposure to drugs. Resistance could not be attributed to mutations in GR or variations in levels of its expression. We conclude that this panel of cell lines provides a suitable in vitro model since it reflects GC resistance in primary ALL.